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and an F cell close together. (b) The Hfr chromosome then begins to pass into the F cell, beginning at the F region of the Hfr chromosome but in the direction away from the F factor. Only a single strand passes into the F cell; this strand and the single strand remaining in the Hfr cell are replicated. After the process is interrupted (c), two crossovers bring the a allele into the F a chromosome (d ).

This plug-in begins by iterating through id if it is an array, recursively calling itself to individually process each element, like this:

The McGraw Hill Companies, 2001

Note This attribute uses the return keyword to stop the server-side function btnSubmitBug_Click()

if (id instanceof Array) { for (var j = 0 ; j < idlength ; ++j) MatrixToText(id[j], msecs) return }

Elie Wollman (1917 ).

(Courtesy of Dr. Elie Wollman and the Pasteur Institute.)

The MT_Flag property of id is then tested If it s true, a call to this plug-in is already operating on the object, so it returns Otherwise, the property is set to true to indicate that a call is in progress on the id, as follows:

Frequency (percent)

from running if a false values is returned from on_Submit().

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After waiting a speci c amount of time, Jacob and Wollman turned the blender on. The spinning motion separated conjugating cells and thereby interrupted their mating. Then the researchers tested the F cells for various alleles originally in the Hfr cell. In an experiment like this, the Hfr strain is usually sensitive to an antibiotic such as streptomycin. After conjugation is interrupted, the cells are plated onto a medium containing the antibiotic, which kills all the Hfr cells. Then the genotypes of the F cells can be determined by replica-plating without fear of contamination by Hfr cells. The mating outlined in table 7.3 was carried out. In the food blender, an Hfr strain sensitive to streptomycin (str s ) but resistant to azide (azi r ), resistant to phage T1 (tonAr ), and prototrophic for the amino acid leucine (leu ) and the sugars galactose ( galB ) and lactose (lac ) was added to an F strain that was resistant to streptomycin (str r ), sensitive to azide (azi s ), sensitive to T1 (tonAs ), and auxotrophic for leucine, galactose, and lactose (leu , galB , and lac ). After a speci c number of minutes (ranging from zero to sixty), the food blender

Next, html is given the HTML contents of id, len its length, and freq the frequency with which the DoMatrixToText() subfunction needs to be called in order for the transition to take msecs milliseconds In addition, the string variable matrix is created, which will hold the random text as it is slowly revealed; count, the counter for each step, is initialized to 0; and chars, the string containing all possible characters for scrambling the text, is populated with the characters a z, A Z and 0 9, as follows:

was turned on.To kill all the Hfr cells, the cell suspension was plated on a medium containing streptomycin. The remaining cells were then plated on medium without leucine. The only colonies that resulted were F recombinants.They must have received the leu allele from the Hfr in order to grow on a medium lacking leucine. Hence, all colonies had been selected to be F recombinants. By replica-plating onto speci c media, investigators were able to determine the azi, tonA, lac, and galB alleles and the percentage of recombinant colonies that had the original Hfr allele (leu ). (Note that by trial and error, it was determined that leucine should be the locus to use to select for recombinants. As we will see, the leucine locus entered rst.) Figure 7.16 shows that as time of mating increases, two things happen. First, new alleles enter the F cells from the Hfr cells. The tonAr allele rst appears among recombinants after about ten minutes of mating, whereas galB rst enters the F cells after about twenty- ve minutes. This suggests a sequential entry of loci into the F cells from the Hfr ( g. 7.17). Second, as time proceeds, the percentage of recombinants with a given allele from the Hfr increases. At ten minutes, tonAr is rst found among recombinants. After fteen minutes, about 40% of recombinants have the tonAr allele from the Hfr; and after about twenty- ve minutes, about 80% of the recombinants have the tonAr allele. This limiting percent-

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