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Sequencing is determining the sequential order of DNA bases in a given piece of DNA Sequencing DNA is relatively easy these days, at least for small pieces (a few thousand nucleotides) In the Sanger dideoxynucleotide method, a specific primer is used that is complementary to one of the two DNA strands you want to sequence The primer can be a vector sequence so that you can sequence any piece of DNA cloned into the vector The primer is a synthetic oligonucleotide that is radiolabeled (or fluorescently labeled) so that you can see all new DNA molecules that have the primer attached to the 5 end Alternatively one of the deoxynucleotides used in the DNA synthesis can be labeled After denaturing the double-stranded DNA that you want to sequence and annealing the primer, the DNA is elongated from the primer (in the 5 to 3 direction) using DNA polymerase The reaction is run for a short time with all four deoxynucleotides There will be pieces of DNA that are at all stages of the replication process the newly synthesized DNA will be of all different lengths The reaction is then stopped by adding it to four separate tubes, each of which contains a different 2 ,3 -dideoxynucleotide When a dideoxynucleotide is incorporated by the polymerase, the elongation stops (there s no 3 -hydroxyl group on the dideoxynucleotide) Alternatively, you can include a small quantity of a dideoxynucleotide during the polymerase reaction so that some DNA stops when a dideoxynucleotide is added and the rest goes on to stop later on The trick is that only one of the four dideoxynucleotides will stop the reaction at any given point in the random mixture of newly synthesized DNA The synthesized DNA is then run on a high-resolution acrylamide gel that can separate DNA molecules that differ in length by one nucleotide Four lanes are run, one for each type of dideoxynucleotide used to stop the reaction A ladder of bands will be seen The shorter bands, at the bottom of the gel, will correspond to termination nearest the primer (near.

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The XS interface is part of the standard Perl distribution; and, as such, it is given more attention here than the third-party SWIG interface XS, or XSUB, stands for eXternal SUBroutine The term XS is usually taken to refer to the interface system, while XSUB specifies an individual function within the XS definition Using the special

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language called XS, you specify the interface between a Perl module and the C functions you want access to The actual layout is not hugely different from a typical C header file, except that the information is organized and structured to include the necessary links for the Perl side of the equation The XS file is then parsed by the xsubpp script, which processes the contents and spits out the C source code ready for compilation The code includes all the necessary glue code and initialization procedures You are expected to supply a suitable module for communicating between Perl and the underlying library; but, otherwise, the process is completed Because of the similarity of the XS file to a header file, there is a tool, h2xs, that will convert existing header files into the XS format This greatly simplifies the process of manually writing an interface to a C library by doing most of the work for you In most cases, the converted file can be passed immediately onto xsubpp, and the resulting C source file is compiled without any further intervention

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the 5 end) The sequence is then read from the bottom (5 end) to the top (3 end) of the gel by noting which dideoxynucleotide stopped the reaction at that length (that is, simply which one of the four lanes has a band in it at that length) (Fig 6-5) Automated methods for doing this are available that can sequence 500 bp in one run and automatically read out the sequence Each type of dideoxynucleotide product is marked with a different color so that all four sequencing reactions can be run in one lane of the gel These automated methods are being used to complete the sequence of the whole human genome

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