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>>> s = StringIO.StringIO() # Create a temp filelike object. >>> p = pickle.Pickler(s,1) # 1 = binary >>> p.dump([1,2,3]) >>> p.dump( Hello! ) >>> s.getvalue() # See the pickled form. ]q\000(K\001K\002K\003e.U\006Hello!q\001. >>> s.seek(0) # Reset the file. >>> u = pickle.Unpickler(s) >>> u.load() [1, 2, 3] >>> u.load() Hello!

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tial and introduction of sample to MS by HPLC or electrophoresis may often facilitate removal of contaminants such as buffer components or chemical agents used in pretreating sample In other situations, it may be desired to introduce the sample directly to MS in a one-dimensional analysis Before analysing proteins or peptides by MS, it is necessary to remove salts present in the sample to avoid the formation of adduct ions with alkali metals These would reduce the abundance of protonated quasimolecular ions This is easily achieved either by dialysis (Section 761), by gel ltration (Section 242) or by reversed phase HPLC of the sample (Section 243) Early studies of peptides using EI/MS and CI/MS involved rendering sample molecules volatile by their extensive derivatization by acylation or esteri cation However, using matrix-based ionization modes, this is no longer necessary.

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